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Vibrational spectroscopy in the electron microscope would be transformative in the study of biological samples, provided that radiation damage could be prevented. However, electron beams typically create high-energy excitations that

Vibrational spectroscopy in the electron microscope would be transformative in the study of biological samples, provided that radiation damage could be prevented. However, electron beams typically create high-energy excitations that severely accelerate sample degradation. Here this major difficulty is overcome using an ‘aloof’ electron beam, positioned tens of nanometres away from the sample: high-energy excitations are suppressed, while vibrational modes of energies <1 eV can be ‘safely’ investigated.

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    Date Created
    • 2016-03-10
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    Identifier
    • Digital object identifier: 10.1038/ncomms10945
    • Identifier Type
      International standard serial number
      Identifier Value
      2041-1723
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    Rez, P., Aoki, T., March, K., Gur, D., Krivanek, O. L., Dellby, N., . . . Cohen, H. (2016). Damage-free vibrational spectroscopy of biological materials in the electron microscope. Nature Communications, 7, 10945. doi:10.1038/ncomms10945

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