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Most molecular fingerprinting techniques, including denaturing gradient gel electrophoresis (DGGE) [1], comparative genomic hybridization (CGH) [2], real-time polymerase chain reaction (RT-PCR) [3], destroy community structure and/or cellular integrity, therefore lost

Most molecular fingerprinting techniques, including denaturing gradient gel electrophoresis (DGGE) [1], comparative genomic hybridization (CGH) [2], real-time polymerase chain reaction (RT-PCR) [3], destroy community structure and/or cellular integrity, therefore lost the info. of the spatial locus and the in situ genomic copy number of the cells. An alternative technique, fluorescence in situ hybridization (FISH) doesn't require sample disintegration but needs to develop specific markers and doesn't provide info. related to genomic copy number.

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    Date Created
    • 2015-06-25
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    Identifier
    • Digital object identifier: 10.1016/j.mex.2015.06.004
    • Identifier Type
      International standard serial number
      Identifier Value
      2215-0161
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    Zhang, P., Valverde, P., Daniel, D., & Fox, P. (2015). Study of a two species microbial community by an inferential comparative genomic analysis tool: Spatial Analytical Microbial Imaging. MethodsX, 2, 331-339. doi:10.1016/j.mex.2015.06.004

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