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The oxidative modification of apolipoprotein A-I’s methionine148 (M148) is associated with defective HDL function in vitro. Multiple reaction monitoring (MRM) is a mass spectrometric technique that can be used to

The oxidative modification of apolipoprotein A-I’s methionine148 (M148) is associated with defective HDL function in vitro. Multiple reaction monitoring (MRM) is a mass spectrometric technique that can be used to quantitate post-translational modifications. In this study, we developed an MRM assay to monitor the abundance ratio of the peptide containing oxidized M148 to the native peptide in ApoA-I. Measurement of the oxidized-to-unoxidized-M148 ratio was reproducible (CV < 5%).

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    Date Created
    • 2014-10-11
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  • Text
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    Identifier
    • Digital object identifier: 10.1016/j.trprot.2014.10.001
    • Identifier Type
      International standard serial number
      Identifier Value
      2212-9626
    • Identifier Type
      International standard serial number
      Identifier Value
      2212-9634
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    Yassine, H. N., Jackson, A. M., Reaven, P. D., Nedelkov, D., Nelson, R. W., Lau, S. S., & Borchers, C. H. (2014). The application of multiple reaction monitoring to assess ApoA-I methionine oxidations in diabetes and cardiovascular disease. Translational Proteomics, 4-5, 18-24. doi:10.1016/j.trprot.2014.10.001

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