There are an increasing variety of applications in which peptides are both synthesized and used attached to solid surfaces. This has created a need for high throughput sequence analysis directly on surfaces. However, common sequencing approaches that can be adapted to surface bound peptides lack the throughput often needed in library-based applications. Here we describe a simple approach for sequence analysis directly on solid surfaces that is both high speed and high throughput, utilizing equipment available in most protein analysis facilities.
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- Digital object identifier: 10.1038/s41598-017-18105-3
- Identifier TypeInternational standard serial numberIdentifier Value2045-2322
- The final version of this article, as published in Scientific Reports, can be viewed online at: http://www.nature.com/articles/s41598-017-18105-3, opens in a new window
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Zhao, Z., Cordovez, L. A., Johnston, S. A., & Woodbury, N. (2017). Peptide Sequencing Directly on Solid Surfaces Using MALDI Mass Spectrometry. Scientific Reports, 7(1). doi:10.1038/s41598-017-18105-3