ASU Scholarship Showcase

Agassiz’s desert tortoise (Gopherus agassizii) is a long-lived species native to the Mojave Desert and is listed as threatened under the US Endangered Species Act. To aid conservation efforts for preserving the genetic diversity of this species, we generated a whole genome reference sequence with an annotation based on deep transcriptome sequences of adult skeletal muscle, lung, brain, and blood. The draft genome assembly for G. agassizii has a scaffold N50 length of 252 kbp and a total length of 2.4 Gbp. Genome annotation reveals 20,172 protein-coding genes in the G. agassizii assembly, and that gene structure is more similar to chicken than other turtles. We provide a series of comparative analyses demonstrating (1) that turtles are among the slowest-evolving genome-enabled reptiles, (2) amino acid changes in genes controlling desert tortoise traits such as shell development, longevity and osmoregulation, and (3) fixed variants across the Gopherus species complex in genes related to desert adaptations, including circadian rhythm and innate immune response. This G. agassizii genome reference and annotation is the first such resource for any tortoise, and will serve as a foundation for future analysis of the genetic basis of adaptations to the desert environment, allow for investigation into genomic factors affecting tortoise health, disease and longevity, and serve as a valuable resource for additional studies in this species complex.

Data Availability: All genomic and transcriptomic sequence files are available from the NIH-NCBI BioProject database (accession numbers PRJNA352725, PRJNA352726, and PRJNA281763). All genome assembly, transcriptome assembly, predicted protein, transcript, genome annotation, repeatmasker, phylogenetic trees, .vcf and GO enrichment files are available on Harvard Dataverse (doi:10.7910/DVN/EH2S9K).

Mexicans and Mexican Americans have resided in Arizona since the early 16th century. Their history, however, is severely under-documented in the state’s archival repositories. As of 2012, this community is represented in a mere 1-2% of the state’s known archival holdings, and 98% of such documentation is held at Arizona State University’s Chicano/a Research Collection (CRC). This article provides a historical review of the CRC’s establishment in 1970 and how its founding Curator, Dr. Christine Marín, transformed a small circulating book collection into Arizona’s largest repository for Mexican American history. It goes on to examine how the CRC’s sitting Archivist is using social media in tandem with a community-based workshop, bilingual promotional materials and finding aids, and description of unprocessed collections as community outreach and collection development tools in order to remedy the under-documentation of Mexican American history in Arizona. We argue that augmenting traditional archival field collecting methods with these strategies enables the CRC to build a more robust relationship with Arizona’s Mexican American community, allows us to continue expanding our archival holdings, and serves as an example for other repositories seeking to enhance their documentation of marginalized communities.