ASU Scholarship Showcase

Agassiz’s desert tortoise (Gopherus agassizii) is a long-lived species native to the Mojave Desert and is listed as threatened under the US Endangered Species Act. To aid conservation efforts for preserving the genetic diversity of this species, we generated a whole genome reference sequence with an annotation based on deep transcriptome sequences of adult skeletal muscle, lung, brain, and blood. The draft genome assembly for G. agassizii has a scaffold N50 length of 252 kbp and a total length of 2.4 Gbp. Genome annotation reveals 20,172 protein-coding genes in the G. agassizii assembly, and that gene structure is more similar to chicken than other turtles. We provide a series of comparative analyses demonstrating (1) that turtles are among the slowest-evolving genome-enabled reptiles, (2) amino acid changes in genes controlling desert tortoise traits such as shell development, longevity and osmoregulation, and (3) fixed variants across the Gopherus species complex in genes related to desert adaptations, including circadian rhythm and innate immune response. This G. agassizii genome reference and annotation is the first such resource for any tortoise, and will serve as a foundation for future analysis of the genetic basis of adaptations to the desert environment, allow for investigation into genomic factors affecting tortoise health, disease and longevity, and serve as a valuable resource for additional studies in this species complex.

Data Availability: All genomic and transcriptomic sequence files are available from the NIH-NCBI BioProject database (accession numbers PRJNA352725, PRJNA352726, and PRJNA281763). All genome assembly, transcriptome assembly, predicted protein, transcript, genome annotation, repeatmasker, phylogenetic trees, .vcf and GO enrichment files are available on Harvard Dataverse (doi:10.7910/DVN/EH2S9K).

As speculative aesthetics, what might feminist ontologies and artist imaginings do for rethinking the "real" assemblages and infrastructures of our quotidian experience, particularly with respect to those that standardize aggressive capital agendas, maximum industrial output and extreme waste? This paper draws connections between female artists' image and event-making, speculative design and tacit knowledge, as sensing tools for technological possibilities at a time when energy dependency, in the form of electricity, is the greatest generator of fossil fuel waste and pollution. I use Remedios Varo's paintings from the mid 1930-1960s as a springboard to think about sustainability and ecological design from an embodied, fem-magic and deep-time perspective, and I draw from other female artists whose work explores technologies and energy, such as Alice Aycock, Tania Candiani, Cassie Meador and Hito Steyerl. An analysis of these artists' works allows me to explore the ways that feminist imaginings function as an ontological orientation that shifts power away from contemporary infrastructures, to decolonize and re-feminize electrical possibilities as alternative ways of engaging with and sensing assemblages.